602 papers
By combining single-nucleosome imaging and super-resolution microscopy, this study reveals that cohesin maintains the integrity of condensed euchromatic domains by preventing local mixing and ensuring transcriptional insulation, thereby refining the traditional view of euchromatin as largely open.
The paper introduces FAβ-gal, an automated, fluorescence-based quantification method that leverages the far-red fluorescence of the X-gal assay's indigo product to provide a sensitive, unbiased, and reproducible measurement of cellular senescence in both cell cultures and tissue sections.
This study demonstrates that the C. elegans proteins SAS-1 and SSNA-1 form dynamic, microtubule-dependent, biomolecular condensates that function as bona fide centriolar satellites, thereby establishing the evolutionary conservation of these structures across species.
This study demonstrates that metformin prevents abdominal aortic aneurysm progression by activating the AMPK-SIRT1-PGC-1α axis to restore vascular smooth muscle cell mitochondrial homeostasis and suppress senescence, a protective mechanism that is entirely dependent on PGC-1α.
This study reveals that a hierarchical tethering mechanism involving LEM-2 and Emerin buffers nuclear envelope assembly against lipid flux imbalances by prioritizing LEM-2 binding sites, thereby preventing membrane invasions and nuclear instability caused by phosphatidylcholine dysregulation.
This study validates the expression of Tau protein in various non-neuronal human and mouse tissues, including the kidney, muscle, and pancreas, suggesting that its dysregulation may contribute to diseases beyond neurodegeneration.
This study identifies Nlrp6 as a critical crypt-intrinsic regulator that coordinates DNA repair and epithelial regeneration by restraining proliferation under genotoxic stress and modulating Csnk2 signaling, thereby preventing genomic instability and colorectal tumorigenesis.
This study demonstrates that the gut microbiota-derived metabolite phenylacetate drives atherosclerosis by inducing endothelial senescence and a secreted IL-6/Notch1 signaling axis that disrupts perivascular adipose tissue function, revealing a novel microbiome-targeted therapeutic avenue for age-related vascular disease.
This study reveals that the zinc metalloprotease ZMPSTE24 interacts with and facilitates the quality control of a normally transient, inverted topological isoform of the antiviral protein IFITM3, which is characterized by a cytosolic C-terminus and hypo-palmitoylation.
This study demonstrates that CRISPR/dCas13-mediated steric interference can specifically disrupt RNA-protein interactions, such as CNBP binding to GA-rich mRNA elements, to reprogram mRNA localization and alter cell motility, providing a tool for investigating the long-term functional consequences of RNA distribution.
This study establishes an FFPE-compatible single-nucleus RNA sequencing method to reveal that Chronic Villitis of Unknown Etiology involves stress-induced trophoblast reprogramming and loss of immune privilege, leading to aberrant maternal-fetal immune activation and adverse pregnancy outcomes.
This paper demonstrates that the Cellular Thermal Shift Assay (CETSA) can be directly applied to unpurified crude reaction mixtures to evaluate cellular target engagement, thereby extending the efficient "direct-to-biology" strategy to the assessment of small-molecule ligands.
This study demonstrates that while IL5 and IL33 both activate human eosinophils, IL33 induces a distinct, transient pear-shaped polarization followed by a flattened, less polarized morphology with reduced motility and increased cell death on periostin and fibrinogen surfaces, highlighting how specific cytokine activators and adhesive substrates jointly regulate eosinophil cytoskeletal reorganization and functional behavior.
This study reveals that cystinosin and its yeast homolog Ers1 function in the early secretory pathway to maintain redox homeostasis, rather than solely acting as lysosomal cystine transporters, thereby providing new insights into the molecular pathology of cystinosis.
Although a direct-to-biology screening approach using 175 compounds failed to identify FBXO22-dependent degraders for four target proteins, it successfully revealed potent FBXO22 self-degrading homo-PROTACs and CRBN- or VHL-mediated FBXO22 degraders.
This study demonstrates that in *Drosophila* neuromuscular junctions, septin complexes (specifically Sep2 and Sep5) act as essential structural organizers that regulate microtubule architecture and synaptic function by buffering microtubule stabilization to preserve pre- and postsynaptic integrity.
This study reveals that short-chain fatty acids and ketone bodies differentially regulate GLP-1 secretion in enteroendocrine cells by activating FFA2 and FFA3 receptors through distinct, non-canonical intracellular signaling pathways that operate independently of or diverge from traditional G-protein mechanisms.
This paper presents a rapid, one-step immunofluorescence strategy using recombinant nanobody-HaloTag constructs to achieve up to eight-target multiplexing in cells and tissues by combining spectral and fluorescence lifetime encoding.
This study utilizes CITE-Seq to demonstrate that while both adult RPESC-derived and PSC-derived RPE cells express key markers, they exhibit distinct molecular and surface protein profiles—specifically higher mature retinal function markers and CD24 in RPESC-RPE versus stem cell-related genes and CD57 in PSC-RPE—suggesting significant differences in their functional and immunomodulatory properties that could influence transplantation outcomes for AMD.
This study demonstrates that the innate immunity receptors CD14 and TLR4 act as tissue-specific co-receptors that, through MyD88-dependent kinase signaling and circadian replenishment, collaborate with other receptors like MerTK and CD36 to regulate the daily peak of photoreceptor outer segment phagocytosis in retinal pigment epithelium cells.